Development of Imaging Probes and Test in Animal Models
The proposed training program will be carried out at the Uni-Torino Platform; each training program will involve small groups of participants (max. 5). The training will be tailored on the background and on the specific requirements each scientist/student will expect from the participation to the proposed activity.
The Torino Training Platform could offer training for scientists and students in various specialities:
- Preparation of Imaging Probes
- In-vitro assessment of physico-chemical properties of MRI Probes
- Set-up of protocols using cell-cultures
- Construction of animal models
Description of work:
- Training in the field of the synthesis of Imaging Probes will be carried out in the laboratories of both the Department of Chemistry IFM at the University of Torino and the LIMA (Laboratorio Integrato di Metodologie Avanzate) at the Bioindustry Park (BIPCa). The latter site is a joined initiative between the University of Torino and BIPCa aimed at improving the links between academy and industry research. Both sites are equipped with advanced analytical facilities (mass spectrometry, UV, FT-IR, NMR, HPLC, Electrophoresis etc). Thematic areas already identified deal with the field of solid-phase synthesis (speciality peptides) and on the synthesis of Imaging Probes based on metal complexes.
- Training in the field of in vitro assessment of the physico-chemical properties of Magnetic Resonance Imaging Probes will be carried out. Training at both basic and advanced levels will be foreseen. The interested scientist/student will be introduced to relaxometry from both an experimental and theoretical point of view. The training will include: i) 1H-relaxometry at fixed frequency and variable magnetic field strenght (Field Cycling Relaxometry) and ii) 17O-relaxometric investigations for the measurement of water exchange rates (a parameter of paramount importance in MRI applications). Two field cycling and one fixed frequency relaxometers are available.
- Training in the use of cell cultures for the assessment of Imaging Probes. Well equipped laboratories for cell cultures are available both at the University of Torino and at LIMA. Training at both basic and advanced levels will be foreseen. The main aims are to train young researchers to the use of cellular models as alternative to animal models in the first steps of the evaluation of novel Imaging Probes.
- Training in the construction of animal models. This activity will be coordinated by Uni-To biologists (Silengo, Altruda) with the contribution of other dedicated groups in EMIL. Training in both basic and advanced levels will be foreseen. Advanced level includes the construction of transgenic and ko mice for dedicated Imaging Probes.