Microscopical research techniques in biomedical applications

Learning objectives

• Gaining insight and experience into the principles of fluorescence microscopy, confocal microscopy (scanning and dual spinning disk), scanning electron microscopy (SEM) and transmission electron microscopy (TEM)
• Gaining insights into the applications in biomedical research
• Getting acquainted with different kinds of preparation techniques

Public

Early stage and experienced researchers from public or private institutions interested in microscopy and its applications in biomedical science

Prerequisits

Basic knowledge on the structure of cells, tissues and organs is recommended

Lectures content and
practical work

Different aspects of modern microscopical techniques will be highlighted and practically dealt with:

• Training on preparation of cryostat sections, ultrathin sections for TEM, SEM-preparations, whole mount preparations
• Training on immunohistochemistry and enzyme histochemistry to visualise neuronal structures with conventional light microscopy, fluorescence microscopy, confocal microscopy and transmission electron microscopy
• Principles and hands on fluorescence microscopy, confocal microscopy, SEM and TEM
• Principles and hands on confocal life cell imaging (UltraVIEW dual spinning disk confocal microscope) including protein trafficking and Ca²⁺-imaging

Training methods

80% integrated practical and 20% theoretical course

Coordination / registrations

Elena CECCARELLI - Corinne CARREAUX,
Phone ++33 1 69 08 25 02
emil-dimi.training@cea.fr

Scientific advisor

Jean-Pierre TIMMERMANS

Course language

English

Duration

5 days

Course location

University of Antwerp (UA), Antwerp, Belgium

Date

March 08-12, 2010

Registration deadline

February 8, 2010

Number of participants

8 participants maximum

Registration fees

DiMI/EMIL/MOLIM members: 100€

public institutions: 500€

private institutions: 800€

Course leader

Inge BROUNS

Phone ++32 32 65 33 00

inge.brouns@ua.ac.be

Key words

Fluorescence microscopy, confocal microscopy, life cell imaging, TEM, SEM